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TABLE I Genes That Contribute to Discrimination of Estrogen Receptor Status

Rank Weight Unigene cluster Estrogen relation

1 0.08 Trefoil factor 1 (pS2) Estrogen induced
2 0.079 Estrogen receptor (ER) 1 ER
3 0.067 Cytochrome P450, subfamily IIB
4 0.064 Trefoil factor 3 Estrogen induced
5 0.061 (Insulin-like growth factor) Estrogen induced
6 0.057 Human clone 23948 mRNA sequence
7 0.056 Microtubule-associated protein tau Estrogen induced
8 0.055 Hepsin
9 0.048 GATA-binding protein 3 Coexpressed with ER
10 0.047 v-myb Avian myeloblastosis viral oncogene homolog Estrogen induced
11 -0.043 Serine proteinase inhibitor, clade B, member 5 (Maspin) Induced by tamoxifen; inverse with ER
12 0.041 N-acetyltransferase 1
13 -0.041 S100 calcium binding protein A9
14 -0.041 Retinoic acid receptor responder 1
15 -0.039 Small inducible cytokine subfamily D, member 1
16 0.039 Hepatocyte nuclear factor 3 Alpha Synergistic with ER
17 0.038 37 kDa Leucine-rich repeat protein
18 0.038 (Androgen receptor) Physical interaction with ER
19 -0.038 Cathepsin C
20 0.037 Inositol polyphosphate-4-phosphatase, type II, 105 kD
21 0.036 Purinergic receptor P2X, ligand-gated ion channel, 4 Estrogen biosynthesis
22 -0.036 KIAA0125 gene product
23 0.036 (Neuropeptide Y receptor Y1)
24 0.035 Meis (mouse) homolog 3
25 0.035 LIV-1 protein Estrogen induced

[Genes are listed according to the discriminatory ranking, with gene 1 having the greatest weight in the discrimination. Negative values indicate an inverse correlation with ER+ status (and thus a positive correlation with ER- status). Reprinted with permission from West M, Blanchette C, Dressman H, Huang E, Ishida S, Spang R, Zuzan H, Olson JA Jr, Marks JR, Nevins JR 2001 Proc Natl Acad Sci 98:11462. Copyright 2001 National Academy of Sciences, U.S.A.]





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