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Section on Obesity, Research Division, Joslin Diabetes Center, and Harvard Medical School, Boston, Massachusetts 02215
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ABSTRACT |
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 TOP ABSTRACT I. BackgroundII. LR Signaling SystemsIII. Leptin Signals in the Regulation of...ACKNOWLEDGEMENTSREFERENCES |
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SHP-2 pathway is a major regulator of extracellular signal-regulated kinase (ERK) activation during leptin signaling in cultured cells, while the Tyr1138 STAT3 pathway induces the feedback inhibitor, suppressor of cytokine signaling 3 (SOCS3), as well as important positive effectors of leptin action. The Jak2-dependent activation of the insulin receptor substrate (IRS) protein phosphatidylinositol 3-kinase (PI3'-K) pathway appears to regulate membrane potential in LRb-expressing neurons and contributes to the regulation of feeding. The Tyr1138 STAT3 pathway mediates transcriptional regulation of the hypothalamic melanocortin pathway in vivo. This pathway is required for the regulation of appetite and energy expenditure by leptin. Interestingly, the Tyr1138 STAT3 pathway does not strongly regulate neuropeptide Y (NPY) and thus is not required for the control of reproduction and growth. Thus, other as-yet-undefined leptin receptor signals are central to these and perhaps other aspects of leptin action. |
I. Background |
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TOPABSTRACTI. BackgroundII. LR Signaling SystemsIII. Leptin Signals in the Regulation of...ACKNOWLEDGEMENTSREFERENCES |
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The predictions of these classic experiments were confirmed when the ob gene was positionally cloned in a large mouse genetics study. The gene encodes a predicted secreted protein, termed leptin (Greek: leptos = thin) (Flier, 1995; Friedman and Halaas, 1998). Soon after the cloning of leptin, it was demonstrated that leptin is synthesized and secreted from adipocytes and that treatment of ob/ob mice with leptin reversed their phenotype (Halaas et al., 1995). Treatment of db/db mice with leptin was ineffective, confirming their insensitivity to this factor.
Following the cloning of leptin, the leptin receptor (LR) was cloned quickly by biochemical techniques employing labeled leptin (Tartaglia et al., 1995). Soon thereafter, the original dbmutation from Jackson Laboratories and several other independently arising db mutations were shown to lie within the leptin receptor gene (lepr) (Chua et al., 1996).
B. LEPTIN COMMUNICATES THE STATUS OF BODY ENERGY STORES TO THE CENTRAL NERVOUS SYSTEM
Adipose cells are the primary source of leptin. Leptin production by fat cells in culture is stimulated by glucocorticoids and indicators of acute nutritional influx such as insulin and inhibited by the counter-regulatory hormones and their intracellular signaling mediators (MacDougald et al., 1995; Rentsch and Chiesi, 1996; Slieker et al., 1996). This regulation of leptin production by insulin (feeding increased insulin increased leptin) and counter-regulatory hormones (fasting increased counter-regulatory hormones decreased leptin) suggests that leptin might serve as an indicator of energy balance (i.e., that increased energy stores yield increased leptin levels). Indeed, circulating leptin levels strongly correlate with body adiposity and changes in acute nutritional status (Frederich et al., 1995; Maffei et al., 1995; Considine et al., 1996).
The stimulation of leptin production by glucocorticoids appears counterintuitive in this light, however, since circulating glucocorticoids are important mediators of the stress and starvation responses. Indeed, while plentiful data suggest that insulin is a critical mediator of increased leptin production in vivo, the diurnal pattern of leptin levels and numerous other data argue against an important role for circulating glucocorticoids in the stimulation of leptin production (Licinio et al., 1997). In contrast, adipocyte-produced autocrine- or paracrine-acting glucocorticoids may be involved, since the production of glucocorticoids by adipocytes increases with the accumulation of triglycerides. Mouse models with increased glucocorticoid production specifically in adipocytes have elevated leptin levels (Bornstein et al., 1997).
Circulating leptin levels thus reflect the status of body energy reserves. Leptin regulates energy balance throughout the body by controlling processes involved in energy intake and utilization. Leptin regulates energy expenditure via the reproductive, growth, autonomic, and other axes of the neuroendocrine system as well as by the immune system (Ahima et al., 1996; Lord et al., 1998) (Figure 1). Thus, in times of inadequate energy stores (e.g., starvation), leptin levels fall; these low leptin levels enhance appetite and decrease energy utilization. Conversely, with adequate energy stores, high leptin levels decrease the drive to eat and permit utilization of energy by the above-mentioned systems.
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C. LEPTIN RECEPTORS AND SITES OF LEPTIN ACTION
Multiple LR isoforms exist; all are products of a single lepr gene and result from alternative mRNA splicing and/or proteolytic processing (Chua et al., 1997; Tartaglia, 1997). LR isoforms fall into three classes: secreted, short, and long. The secreted forms are alternative splice products (e.g., the murine LRe) or proteolytic cleavage products of membrane-bound LR forms: These contain only extracellular leptin-binding domains and complex with circulating leptin, perhaps regulating free leptin concentrations (Ge et al., 2002). Short- (e.g., LRa) and long-form (LRb) receptors contain identical extracellular and transmembrane domains as well as the same first 29 intracellular amino acids and diverge in sequence secondary to alternative splicing of 3' exons. LRb is highly conserved among species and possesses an intracellular domain of approximately 300 residues (Chua et al., 1997; Tartaglia, 1997).
While the function of the short LR forms remains unclear, LRb is critical for leptin action. Indeed, the originally described db/db mice lack only LRb (as a consequence of a mutation that results in mis-splicing of the LRb message) but exhibit a phenotype indistinguishable from that of leptin-deficient ob/ob animals and of db3J/db3J mice (which are deficient in all LR isoforms) (Chua et al., 1997; Tartaglia, 1997; Friedman and Halaas, 1998).
Much of leptin’s action is attributable to effects in the central nervous system (CNS), especially in the basomedial hypothalamus, the site of highest LRb expression (Elmquist et al., 1998,1999). Here, leptin acts on neurons that regulate levels of circulating hormones (e.g., thyroid hormone, sex steroids, growth hormone) (Elmquist et al., 1999; Inui, 1999). Leptin action on these hypothalamic neurons also regulates the activity of the autonomic nervous system, although direct leptin action on brainstem LRb-expressing neurons may play a role in this and other leptin actions (Elmquist et al., 1997; Grill et al., 2002). Leptin action on the immune system appears to result from direct action on LRb-expressing T cells (Lord et al., 1998). Leptin also may regulate glucose homeostasis (independently of effects on adiposity). Leptin regulates glycemia at least partly via the CNS but also may directly regulate some metabolic tissues (Liu et al., 1988; Kieffer et al., 1997; Kulkarni et al., 1997; Burcelin et al., 1999).
D. LEPTIN RESISTANCE AND OBESITY
Over one quarter of adult Americans are obese. The incidence of obesity continues to rise in the United States as well as in other industrialized nations. Obesity is a major risk factor for type 2 diabetes, cardiovascular disease, and some forms of cancer (Roth, 1998). Since administration of leptin to rodents decreases food intake and increases energy expenditure, resulting in loss of fat mass, leptin initially was hailed as a potential cure for obesity (Tartaglia, 1997; Elmquist et al., 1998,1999; Friedman and Halaas, 1998; Inui, 1999).
With the exception of rare human patients with genetic leptin deficiency, however, circulating leptin levels correlate well with body mass index (BMI) and total body fat mass. Hence, obese individuals have elevated circulating leptin levels but this abundant leptin fails to mediate weight loss, suggesting that most human obesity represents a form of leptin resistance. Indeed, although therapy with exogenous leptin does augment weight loss, the effects of leptin are modest at the doses that have been tested. A number of potential mechanisms have been postulated to underlie leptin resistance, including defects in leptin access into the brain, in LRb signaling, or in pathways/neurons that mediate downstream leptin action. We predict that defects in LRb signaling or downstream effectors will underlie leptin resistance, since leptin may gain direct access (without need for transport across the blood-brain barrier) to neurons in the basomedial hypothalamus that are important for the regulation of energy balance.
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II. LR Signaling Systems |
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TOPABSTRACTI. BackgroundII. LR Signaling SystemsIII. Leptin Signals in the Regulation of...ACKNOWLEDGEMENTSREFERENCES |
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The homology of LRb with other type I cytokine receptors (e.g., the gp 130 subunit of the interleukin (IL)-6 receptor family) suggests a number of signaling functions that were tested quickly by a number of groups: leptin binding to LRb activates Jak kinases and mediates cytokine receptor-like signals, including those mediated by the latent transcription factor, signal transducer and activator of transcription 3 (STAT3) (Baumann et al., 1996; Tartaglia, 1997; White et al., 1997). A specific tyrosine residue (Tyr1138) in the intracellular domain of LRb mediates the activation of STAT3.
We felt that LRb was likely to mediate other important signals as well and set out to identify them. In general, tyrosine kinase-based signaling is mediated by the recruitment of signaling molecules to tyrosine-phosphorylated motifs on the receptor and/or tyrosine kinase (Koch et al., 1991) — in this case, on the intracellular domain of LRb and its associated Jak kinase(s). This recruitment of downstream signaling proteins generally is mediated by specialized phosphotyrosine binding domains (e.g., src homology 2 (SH2) domains) in the signaling proteins. These SH2 domains generally require the presence of phosphotyrosine in the target protein for high-affinity binding but also recognize surrounding amino acid residues. Thus, each tyrosine phosphorylation site on a receptor recruits specific SH2 domain-containing proteins, based upon the amino acid motif in which the phosphotyrosine residue is located. In this manner, the residues Met-Pro-Gln (MPQ) following Tyr1138 of LRb dictate binding of STAT3 when Tyr1138 becomes phosphorylated (Baumann et al., 1996; Tartaglia, 1997; White et al., 1997).
Understanding the signals mediated by LRb requires identifying the tyrosine residues within the LRb/Jak kinase complex that are phosphorylated during signaling and subsequently determining the signaling proteins that interact with these sites. While the identification of LRb phosphorylation sites should be relatively straightforward, identifying phosphorylation sites on the associated Jak kinase is more complicated, given that it requires identifying the Jak kinase isoform(s) utilized by LRb (Ihle, 1995) as well as sorting through a greater number of tyrosine phosphorylation sites (Feng et al., 1997). Furthermore, in order to understand the failure of short LR isoforms to compensate for the lack of LRb in db/db animals, it is critical to define their ability to mediate Jak-dependent signaling.
In order to avoid potential artifacts introduced by the endogenous (mostly short-form) LR isoforms expressed in most cells, we employ a chimeric receptor (ELR) that contains the extracellular ligand-binding domain of the erythropoeitin (Epo) receptor fused to the transmembrane and intracellular domains of LR forms (Banks et al., 2000). The EpoR is expressed on few cells or cell lines, so ELR permits us to use Epo to stimulate signaling via various LR intracellular domains without triggering endogenous receptors.
B. JAK KINASE SELECTIVITY AND UTILIZATION BY LR ISOFORMS
There are four known members of the Jak family: Jak1–3 and Tyk2. Jak1, Jak2, and Tyk2 are widely expressed, whereas Jak3 is found only in cells of the hematopoeitic/immune systems (Ihle, 1995). We expressed ELR in 32D myeloid progenitor cells and assessed its ability to mediate the activation of the commonly expressed Jak kinases, using the endogenous (Jak-promiscuous) IL-3 receptor as a positive control for the activation of each kinase. This analysis demonstrated that the intracellular domain of ELR activated only Jak2, not Jak1 or Tyk2 (Kloek et al., 2002). Furthermore, while disruption of Jak2 expression in fibrosarcoma cells abrogated ELR signaling, disruption of Jak1 or Tyk2 had no effect. Thus, Jak2 represents the unique Jak kinase involved in signaling by the intracellular domain of LRb.
All functional cytokine receptors contain a proline-rich "Box 1" motif that is required for Jak kinase interaction and activation. Additional less-conserved sequences (sometimes referred to as "Box 2") COOH-terminal to Box 1 are also important for Jak kinase interactions and likely function in Jak kinase isoform selectivity (Ihle and Kerr, 1995; Taga and Kishimoto, 1997). Our deletion analysis demonstrated that, in addition to Box 1 sequences, intracellular residues 31–36 of LRb (i.e., immediately downstream of the alternative splice junction following amino acid 29) are required for Jak2 activation (Kloek et al., 2002). Interestingly, we observed that high-level overexpression of Jak kinases by transient transfection could decrease the stringency of the requirement for residues 31–36, consistent with the ability of others to observe (relatively weak) LRb-Jak1 and LRa-Jak2 signaling under transient transfection conditions (Bjorbaek et al., 1997). These results suggests a model in which Box 1 is absolutely required for all cytokine receptor-Jak kinase interactions, with the more-flexible role (at least under experimental conditions) for residues homologous to intracellular amino acids 31–36 of LRb in determining the specificity of the Jak kinase that interacts with a particular receptor. Indeed, homology between the Box 2 regions of LRb and other Jak2-associated cytokine receptors suggests that a loosely conserved E/N-X0–2E/N-X0–2L/I motif mediates Jak2 association (Kloek et al., 2002).
This motif is absent from all described short LR isoforms, explaining the inability of these molecules to mediate leptin action in db/db animals (Tartaglia, 1997; White et al., 1997; Kloek et al., 2002). The lack of Jak2 signaling by short LR isoforms suggests we cannot rule out, on the basis of the failure of these receptors to transmit any leptin signal, the possibility that Jak2 may be capable of mediating some physiologic leptin actions in the absence of LRb tyrosine phosphorylation sites.
C. LRb TYROSINE PHOSPHORYLATION
There are three conserved tyrosine residues on the intracellular domain of LRb: Tyr985, Tyr1077, and Tyr1138 (Tartaglia, 1997; White et al., 1997; Banks et al., 2000). In the human long-form LR, two additional intracellular tyrosine residues exist, albeit within motifs that do not appear to be particularly hydrophilic and accessible to kinases (Tartaglia et al., 1995). In order to determine whether any of these sites might be candidates for phosphorylation, we generated a bacterial fusion protein containing all of the tyrosine residues of the intracellular domain of the human long-form LR (except the Tyr1138 homologue) and subjected it to phosphorylation with recombinant Jak2. Analysis of the resulting phosphorylated glutathione-S-transferase (GST)-LR demonstrated tyrosine phosphorylation of the Tyr985 homologue but not of any other sites, suggesting that Tyr985 and Tyr1138 might be the unique sites of LRb tyrosine phosphorylation (M.G. Myers, Jr., unpublished observations).
In order to examine the phosphorylation of tyrosine residues on the intracellular tail of LRb in intact cells, we mutated the three LRb tyrosines individually and in combination and examined the phosphorylation of the mutant receptors during signaling in 293 cells (Banks et al., 2000). This analysis confirmed that Tyr985 and Tyr1138 are phosphorylated during receptor signaling but Tyr1077 is not phosphorylated and does not contribute to signaling. Thus, three primary intracellular signaling pathways emanate from LRb (Figure 2): those originating directly from Jak2 tyrosine phosphorylation sites, from Tyr985 of LRb, and from Tyr1138 of LRb.
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The canonical mechanism by which tyrosine kinases activate ERK is via the SH2 domain-containing adapter protein, growth factor receptor binding (Grb)-2. Grb-2 recruitment by tyrosine kinase signaling complexes mediates the activation of the small G protein, p21ras, to stimulate the upstream activators of ERK (Lowenstein et al., 1992; Myers et al., 1994; Banks et al., 2000). In order to understand the mechanism of ERK activation by Tyr985, we examined tyrosyl phosphoproteins associated with the adapter protein, Grb-2, during ELR signaling and discovered an 
75-kDa Grb-2-associated tyrosyl phosphoprotein (Banks et al., 2000). Taking a candidate approach, we showed that this 75-kDa protein is identical to the SH2 domain-containing tyrosine phosphatase SHP-2 and that Tyr985 directly recruits SHP-2 via its COOH-terminal SH2 domain (Banks et al., 2000; Bjorbaek et al., 2001; Kloek et al., 2002). Indeed, the YATL motif in which Tyr985 lies is predicted to bind to the COOH-terminal SH2 domain of SHP-2 (Songyang et al., 1993; De Souza et al., 2002). Thus, phosphorylation of LRb Tyr985 mediates the recruitment of SHP-2, which is then phosphorylated and, in turn, recruits Grb-2 to mediate activation of the p21ras ERK cascade.
E. SIGNALING VIA TYR1138 STAT3 MEDIATES TRANSCRIPTION OF SOCS3 AND FEEDBACK INHIBITION ON LRb SIGNALING
The role of Tyr1138 phosphorylation in recruiting STAT3 to the LRb/Jak2 complex was suggested by homology between LRb and other cytokine receptors of the IL-6 receptor family. Indeed, mutation of this site abrogates STAT3 signaling by LRb (Baumann et al., 1996; Tartaglia, 1997; White et al., 1997). We thus utilized receptors mutant for Tyr1138 to investigate potential roles for STAT3 in the regulation of gene expression, demonstrating that this pathway induces expression of SOCS3, which is induced by prolonged LRb activation and mediates feedback inhibition of LRb signaling (Bjorbaek et al., 1998,2000; Banks et al., 2000). We furthermore examined the mechanism by which SOCS3 mediates feedback inhibition on signaling by the intracellular domain of LRb. We demonstrated that SOCS3 interacts via its SH2 domain with phosphorylated Tyr985 of LRb and that sensitive blockade of LRb STAT3 signaling is mediated by this interaction (Bjorbaek et al., 2000). Our recent unpublished results suggest that another LRb STAT3-dependent mechanism of feedback inhibition of LRb signaling may directly block Jak2 activation.
F. SIGNALS MEDIATED BY JAK2 AND FUTURE DIRECTIONS IN LRb SIGNALING
Jak2 tyrosine phosphorylation during LRb stimulation mediates some signals independently of tyrosine phosphorylation sites on LRb (e.g., a portion of ERK activation, IRS protein phosphorylation) (Banks et al., 2000). Unfortunately, most Jak2 tyrosine phosphorylation sites have not been defined, impairing our understanding of the mechanisms by which Jak2-dependent signals are mediated. Identifying tyrosine phosphorylation sites on Jak2 and defining their roles in intracellular signaling will be critical to understanding the breadth and depth of LRb signaling.
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III. Leptin Signals in the Regulation of Mammalian Physiology |
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TOPABSTRACTI. BackgroundII. LR Signaling SystemsIII. Leptin Signals in the Regulation of...ACKNOWLEDGEMENTSREFERENCES |
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Within the nuclei of the basomedial hypothalamus, LRb is expressed at its highest levels in the ARC. Within the ARC, LRb is found in at least two distinct populations of neurons (Figure 3): 1) neurons that coexpress neuropeptide Y (NPY) and agouti-related peptide (AgRP) and 2) neurons that express pro-opiomelanocortin (POMC) (Elmquist et al., 1999; Schwartz et al., 2000). POMC is processed to alpha melanocyte-stimulating hormone (
MSH) in the LRb/POMC neuron. MSH mediates a powerful anorectic (appetite-suppressing) signal; LRb stimulates the expression of POMC and activates the LRb/POMC neuron (Schwartz et al., 2000; Cowley et al., 2001). AgRP is an antagonist of MSH signaling and NPY is itself an orexigenic (appetite-stimulating) hormone that also acts to suppress the central LRb growth and reproductive axes (Erickson et al., 1996b; Fan et al., 1997; Seeley et al., 1997; Bates et al., 2003). Leptin acts via LRb to inhibit the NPY/AgRP neurons and to suppress expression of these neuropeptides. Thus, LRb signaling stimulates the production of anorectic neuropeptides and suppresses levels of orexigenic peptides. Conversely, when leptin action is decreased or deficient (e.g., starvation, ob/ob or db/db mice), appetite is stimulated via the suppression of anorectic neuropeptides (e.g., POMC) and by increased expression of orexigenic peptides (e.g., NPY, AgRP) (Elmquist et al., 1999; Schwartz et al., 2000). LRb-expressing ARC NPY/AgRP and/or POMC neurons also regulate energy expenditure and other elements of neuroendocrine function (Ahima et al., 1996). Other distinct populations of LRb-expressing neurons may be found in the ARC (Jureus et al., 2000). The neurochemical properties of LRb-expressing neurons in the DMR, VHM, and elsewhere (including the brainstem) are poorly defined.
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First described as insulin receptor substrates, the IRS proteins (IRS 1–4) are members of a class of intracellular signaling molecules termed docking proteins that are phosphorylated by a number of activated tyrosine kinases (e.g., insulin receptor, some cytokine receptors) (Myers and White, 2002). Docking proteins, including the IRS proteins, are devoid of enzymatic activity but are phosphorylated on multiple tyrosine residues to mediate SH2-protein recruitment and downstream signaling. While IRS proteins contain tyrosine phosphorylation sites in numerous motifs that recruit several different SH2-proteins, the majority of sites lie in YMXM motifs that bind and activate PI3'-K.
The first, albeit indirect, evidence for a potential role of the IRS protein PI3'-K pathway in leptin action came from the phenotype of the IRS-2 null (IRS-2-/-) mouse (Withers et al., 1998). In addition to other defects, IRS-2-/- animals display increased feeding and decreased metabolic rate in the presence of increased adiposity and circulating leptin, suggesting functional leptin resistance (although not as severe as in db/db animals). No such phenotype has been noted in animals null for any of the other three IRS proteins (Araki et al., 1994).
Blockade of PI3'-K activity abrogates leptin-mediated hyperpolarization and inhibition of (presumably) LRb/NPY/AgRP hypothalamic neurons (Spanswick et al., 1997b; Harvey et al., 2000). Furthermore, leptin stimulates IRS-2-associated PI3'-K activity in the hypothalamus and pharmacological blockade of PI3'-K activity in the hypothalamus blocks the anorectic effect of leptin in vivo (Niswender et al., 2001).
Importantly, inhibition of PI3'-K does not alter the anorectic effect of melanocortin agonists that operate downstream of LRb neurons, suggesting that this effect of PI3'-K blockade is specific for LRb neurons. PI3'-K activity also is required for leptin-regulated sympathetic nervous system function (Rahmouni et al., 2003).
As in vivo (Niswender et al., 2001), we observe that LRb stimulation mediates the tyrosine phosphorylation of IRS proteins and activation of the PI3'-K pathway in cultured cells. This signal is mediated by Jak2 independently of tyrosine phosphorylation sites on LRb (M.G. Myers, Jr., et al., unpublished observations).
The recruitment of IRS proteins/PI3'-K by Jak2 is less robust than by the insulin receptor, however. Indeed, animals with decreased expression of neuronal insulin receptors (NIRKO mice) display a modest obesity phenotype, similar to the phenotype of IRS-2-/- mice (Bruning et al., 2000). Also, insulin activates the IRS-2 PI3'-K pathway in the hypothalamus and PI3'-K activity is required for the anorectic activity of insulin in the brain (Niswender et al., 2003). Thus, both leptin and insulin stimulate hypothalamic IRS-2 PI3'-K signaling and both require PI3'-K activity for their anorectic functions. Although the relative contribution of insulin- vs. leptin-stimulated PI3'-K to functional (anorectic) hypothalamic signaling remains difficult to dissect, the importance of the IRS protein PI3'-K pathway is clear, as will be the understanding of the potential role of this pathway in functional leptin resistance. It will be interesting to determine the neuropeptide phenotype of the neuronal population(s) in which PI3'-K activity is required for the anorectic actions of leptin and insulin as well as the role of PI3'-K in other leptin functions (e.g., neuroendocrine and immune regulation).
C. LRb STAT3 SIGNALING IN THE CONTROL OF PHYSIOLOGY BY LEPTIN
We have directly addressed the contribution of the LRb STAT3 pathway to physiology by studying homologously targeted "knock-in" mice in which LRb is replaced by a mutant molecule (LRbS1138) that contains a substitution mutation of Tyr1138 (the STAT3 binding site) (Bates et al., 2003). While LRbS1138 fails to mediate activation of STAT3 during leptin signaling, this mutant regulates all other LRb signaling pathways normally. This approach ensures that the expression pattern and levels of LRbS1138 mirror that of wild-type LRb, so alterations in physiology in the resulting animals are secondary to lack of LRb STAT3 signaling rather than due to a failure of correct LRb expression. Like db/db animals, mice homozygous for LRbS1138 (s/s) display hyperphagia and decreased energy expenditure, resulting in massive, early-onset obesity associated with increased serum leptin levels. Thus, the LRb STAT3 signal is central to the regulation of body weight by leptin and dysfunction of this signal generates leptin resistance.
Important differences exist between the phenotypes of s/s mice and db/db mice, however (Bates et al., 2003). While db/db animals are infertile and demonstrate decreased linear growth, s/s mice are fertile and demonstrate increased linear growth, compared to wild-type animals.
In order to understand the neurochemical basis for the s/s phenotype, we analyzed hypothalamic neuropeptide expression, revealing that, like db/db mice, s/s mice have decreased POMC and increased AgRP mRNA levels in the hypothalamus. In contrast, while db/db animals display dramatic induction of hypothalamic NPY mRNA, levels of NPY message are near normal in s/s animals. These data suggest that LRb STAT3 signaling is a critical regulator of hypothalamic melanocortin action and that dysregulated melanocortin signaling (as opposed to alterations in NPY) accounts for the obesity of s/s animals. Additionally, non-STAT3 LRb signals are critical regulators of NPY expression in the LRb/NPY neuron.
These results are consistent with the proposed role for NPY in suppressing the hypothalamic growth and gonadal axes; thus, the increased NPY signaling in ob/ob and db/db mice may only modestly increase feeding but may be primarily responsible for infertility and growth retardation in these mouse models. Indeed, the phenotype of ob/ob mice null for NPY (ob/ob,Npy-/-) displays important similarities with the s/s phenotype (Erickson et al., 1996a): both show improved function of the hypothalamic/gonadal axis and increased linear growth with only modestly attenuated obesity, compared to ob/ob and db/db mice. The increased linear growth observed in these models likely results from the absolute lack of NPY in ob/ob,Npy-/- animals and the hyperactivation of non-STAT3 LRb signals (e.g., signals that inhibit the release of NPY) in hyperleptinemic s/s animals, as is observed in most forms of human obesity. Thus, functional leptin resistance in humans could stem from inhibition of pathways controlled by LRb STAT3 action.
Returning to the concept of leptin resistance in common forms of obesity, one would expect that dysregulation of the NPY pathway would result in neuroendocrine abnormalities (e.g., infertility, growth retardation) that are not generally observed in obesity. In contrast, alterations in STAT3-mediated pathways (e.g., melanocortin action) could generate a phenotype of impaired energy balance coupled with relatively normal neuroendocrine function.
D. FUTURE DIRECTIONS IN LRb SIGNALING AND PHYSIOLOGY
Our data from s/s animals demonstrate that LRb-STAT3 signaling is critical for the transcriptional regulation of melanocortin action in the ARC but not for the regulation of NPY. Additionally, STAT3 is not likely to be involved in the regulation of membrane potential in ARC neurons, as leptin-regulated membrane potential is too rapid to be transcriptionally mediated (Spanswick et al., 1997b; Cowley et al., 2001).
Note that the phenotype of the s/s animals does not suggest the physiologic irrelevance of non-STAT3 pathways to the control of feeding and energy expenditure, only that STAT3 signaling is important for the regulation of energy homeostasis. We suggest that the Jak2 IRS protein PI3'-K pathway represents a major STAT3-independent mediator of LRb action. Data from numerous laboratories suggest that PI3'-K action regulates membrane potential in the LRb/NPY neuron (Spanswick et al., 1997a; Harvey et al., 2000). PI3'-K action similarly may control membrane potential in the LRb/POMC neuron. Although this possibility remains untested, one reasonable hypothesis is that PI3'-K action may control the repression of NPY by LRb. However, it is also possible that Tyr985/SHP-2-mediated signals or other uncharacterized signals mediated via Jak2 tyrosine phosphorylation sites may control NPY expression and/or membrane potential. The analysis of Jak2 and Tyr985-mediated signals in LRb action in vitro and in vivo will represent an important next step in understanding the mechanisms by which LRb regulates mammalian physiology.
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ACKNOWLEDGEMENTS |
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TOPABSTRACTI. BackgroundII. LR Signaling SystemsIII. Leptin Signals in the Regulation of...ACKNOWLEDGEMENTSREFERENCES |
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TOPABSTRACTI. BackgroundII. LR Signaling SystemsIII. Leptin Signals in the Regulation of...ACKNOWLEDGEMENTSREFERENCES |
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 P. F. Bodary Links Between Adipose Tissue and Thrombosis in the Mouse Arterioscler. Thromb. Vasc. Biol., November 1, 2007; 27(11): 2284 - 2291. [Abstract] [Full Text] [PDF]
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 Y. Gong, R. Ishida-Takahashi, E. C. Villanueva, D. C. Fingar, H. Munzberg, and M. G. Myers Jr. The Long Form of the Leptin Receptor Regulates STAT5 and Ribosomal Protein S6 via Alternate Mechanisms J. Biol. Chem., October 19, 2007; 282(42): 31019 - 31027. [Abstract] [Full Text] [PDF]
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 S. R. Thorn, M. J. Meyer, M. E. Van Amburgh, and Y. R. Boisclair Effect of Estrogen on Leptin and Expression of Leptin Receptor Transcripts in Prepubertal Dairy Heifers J Dairy Sci, August 1, 2007; 90(8): 3742 - 3750. [Abstract] [Full Text] [PDF]
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 K. N. Lai, S. C.W. Tang, and J. C.K. Leung MEDIATORS OF INFLAMMATION AND FIBROSIS Perit. Dial. Int., June 1, 2007; 27(Supplement_2): S65 - S71. [Abstract] [Full Text] [PDF]
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H. Munzberg, E. E. Jobst, S. H. Bates, J. Jones, E. Villanueva, R. Leshan, M. Bjornholm, J. Elmquist, M. Sleeman, M. A. Cowley, et al. Appropriate Inhibition of Orexigenic Hypothalamic Arcuate Nucleus Neurons Independently of Leptin Receptor/STAT3 Signaling J. Neurosci., January 3, 2007; 27(1): 69 - 74. [Abstract] [Full Text] [PDF]
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C. D. Morrison, C. L. White, Z. Wang, S.-Y. Lee, D. S. Lawrence, W. T. Cefalu, Z.-Y. Zhang, and T. W. Gettys Increased Hypothalamic Protein Tyrosine Phosphatase 1B Contributes to Leptin Resistance with Age Endocrinology, January 1, 2007; 148(1): 433 - 440. [Abstract] [Full Text] [PDF]
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 H. Cui, F. Cai, and D. D. Belsham Leptin signaling in neurotensin neurons involves STAT, MAP kinases ERK1/2, and p38 through c-Fos and ATF1 FASEB J, December 1, 2006; 20(14): 2654 - 2656. [Abstract] [Full Text] [PDF]
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 C. B. Kaelin, L. Gong, A. W. Xu, F. Yao, K. Hockman, G. J. Morton, M. W. Schwartz, G. S. Barsh, and R. G. MacKenzie Signal Transducer and Activator of Transcription (Stat) Binding Sites But Not Stat3 Are Required for Fasting-Induced Transcription of Agouti-Related Protein Messenger Ribonucleic Acid Mol. Endocrinol., October 1, 2006; 20(10): 2591 - 2602. [Abstract] [Full Text] [PDF]
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A. Tups, M. Helwig, S. Stohr, P. Barrett, J. G. Mercer, and M. Klingenspor Photoperiodic regulation of insulin receptor mRNA and intracellular insulin signaling in the arcuate nucleus of the Siberian hamster, Phodopus sungorus Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R643 - R650. [Abstract] [Full Text] [PDF]
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R. Ishida-Takahashi, F. Rosario, Y. Gong, K. Kopp, Z. Stancheva, X. Chen, E. P. Feener, and M. G. Myers Jr. Phosphorylation of jak2 on ser523 inhibits jak2-dependent leptin receptor signaling. Mol. Cell. Biol., June 1, 2006; 26(11): 4063 - 4073. [Abstract] [Full Text] [PDF]
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S. R. Thorn, S. Purup, W. S. Cohick, M. Vestergaard, K. Sejrsen, and Y. R. Boisclair Leptin Does Not Act Directly on Mammary Epithelial Cells in Prepubertal Dairy Heifers J Dairy Sci, May 1, 2006; 89(5): 1467 - 1477. [Abstract] [Full Text] [PDF]
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 M. C. Henson and V. D. Castracane Leptin in Pregnancy: An Update Biol Reprod, February 1, 2006; 74(2): 218 - 229. [Abstract] [Full Text] [PDF]
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K. Laubner, T. J. Kieffer, N. T. Lam, X. Niu, F. Jakob, and J. Seufert Inhibition of Preproinsulin Gene Expression by Leptin Induction of Suppressor of Cytokine Signaling 3 in Pancreatic {beta}-Cells Diabetes, December 1, 2005; 54(12): 3410 - 3417. [Abstract] [Full Text] [PDF]
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 M. B. Chen, A. J. McAinch, S. L. Macaulay, L. A. Castelli, P. E. O'Brien, J. B. Dixon, D. Cameron-Smith, B. E. Kemp, and G. R. Steinberg Impaired Activation of AMP-Kinase and Fatty Acid Oxidation by Globular Adiponectin in Cultured Human Skeletal Muscle of Obese Type 2 Diabetics J. Clin. Endocrinol. Metab., June 1, 2005; 90(6): 3665 - 3672. [Abstract] [Full Text] [PDF]
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R. Gutierrez-Juarez, S. Obici, and L. Rossetti Melanocortin-independent Effects of Leptin on Hepatic Glucose Fluxes J. Biol. Chem., November 26, 2004; 279(48): 49704 - 49715. [Abstract] [Full Text] [PDF]
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D. E. Edwards, R. P. Bohm Jr, J. Purcell, M. S. Ratterree, K. F. Swan, V. D. Castracane, and M. C. Henson Two Isoforms of the Leptin Receptor Are Enhanced in Pregnancy-Specific Tissues and Soluble Leptin Receptor Is Enhanced in Maternal Serum with Advancing Gestation in the Baboon Biol Reprod, November 1, 2004; 71(5): 1746 - 1752. [Abstract] [Full Text] [PDF]
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